Journal: Scientific reports

Article Title: Primary mouse myoblast metabotropic purinoceptor profiles and calcium signalling differ with their muscle origin and are altered in mdx dystrophinopathy.

doi: 10.1038/s41598-023-36545-y

Figure Lengend Snippet: Figure 1. P2RYs transcript levels in myoblasts derived from Tibialis anterior (TA), Gastrocnemius (GC), Soleus (SOL) and Flexor Digitorum Brevis (FDB) muscles. Transcripts encoding P2RY2, P2RY4 and P2RY6 were tested in the same sample, thus their relative expression levels can be compared quantitatively., mRNAs encoding two ADP-activated receptors (P2RY1, P2RY12) were also detected in these samples. *p < 0.05, **p < 0.01 (mdx vs. w/t).

Article Snippet: The following primary antibodies were used: P2RY1 (1:270, APR-009), P2RY2 (1:270, APR-010), P2RY4 (1:300, APR-006), P2RY6 (1:250, APR-011), P2RY12 (1:270, APR-012), P2RY13 (1:270, APR017); all Alomone Labs, calsequestrin (ab126241), calreticulin (ab128885), SERCA1 (ab124501) and SERCA2 (ab91032); all Abcam, diluted 1:1000, Gαq11 (06-709 Merck Millipore, 1:1000,), PLCβ isoforms 3–4 (sc-133231, sc-166131, respectively; Santa Cruz Biotechnology, all diluted 1:100,), NCX1 (R3F1 Swant, 1:1000), NCX3 (ab84708 Abcam, 1:500), PMCA (ab2825 Abcam, 1:1000) and IP3R (#8568 Cell Signalling Technology, 1:1000).

Techniques: Derivative Assay, Muscles, Expressing

Journal: Scientific reports

Article Title: Primary mouse myoblast metabotropic purinoceptor profiles and calcium signalling differ with their muscle origin and are altered in mdx dystrophinopathy.

doi: 10.1038/s41598-023-36545-y

Figure Lengend Snippet: Figure 4. Western bloting analysis of ADP-activated metabotropic receptors P2RY12 and P2RY13. Each bar shows Western blot data from 3 independent experiments ± SD. Representative western blots are also shown. *p < 0.05 (mdx vs. w/t).

Article Snippet: The following primary antibodies were used: P2RY1 (1:270, APR-009), P2RY2 (1:270, APR-010), P2RY4 (1:300, APR-006), P2RY6 (1:250, APR-011), P2RY12 (1:270, APR-012), P2RY13 (1:270, APR017); all Alomone Labs, calsequestrin (ab126241), calreticulin (ab128885), SERCA1 (ab124501) and SERCA2 (ab91032); all Abcam, diluted 1:1000, Gαq11 (06-709 Merck Millipore, 1:1000,), PLCβ isoforms 3–4 (sc-133231, sc-166131, respectively; Santa Cruz Biotechnology, all diluted 1:100,), NCX1 (R3F1 Swant, 1:1000), NCX3 (ab84708 Abcam, 1:500), PMCA (ab2825 Abcam, 1:1000) and IP3R (#8568 Cell Signalling Technology, 1:1000).

Techniques: Western Blot

Journal: iScience

Article Title: Sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids

doi: 10.1016/j.isci.2024.109628

Figure Lengend Snippet:

Article Snippet: Rabbit anti-P2Y12 , Atlas Antibodies , Cat# HPA014518; RRID: AB_2669027.

Techniques: Virus, Recombinant, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Software

Journal: Glia

Article Title: Sexual dimorphism in the inflammatory response to traumatic brain injury

doi: 10.1002/glia.23171

Figure Lengend Snippet: Representative images indicate cortical brain sections stained with P2Y12 (resident microglial cells, red) and F4/80 (macrophages, green) markers, and nuclei counterstained with dapi (blue). No F4/80 positive cells were observed in sham (Sh) male mice (A, A1, A2, a3), sham female mice (B, B1, B2, b3), 4h TBI male mice (C, C1, C2, c3), or 4h TBI female mice (D, D1, D2, d3). F4/80 positive macrophages with amoeboid morphology predominantly appear at 1 dpi in the perilesional cortex of male mice (E, E1, E2, e3) but remain absent in female brains (F, F1, F2, f3). F4/80 positive macrophages persist at 3 dpi in male TBI brain (G, G1, G2, g3) but still remain absent in female TBI brain (H, H1, H2). By 7 dpi F4/80 cells are widespread in the injured cortex of male mice (I, I1, I2, i3), and are apparent around the lesion in female TBI brain (J, J1, J2, j3). Quantitative graphs indicate an increase in F4/80 positive macrophages (K) and CD68 positive activated microglia/macrophages cells (L) in males compared to females at all time-points. Representative images of CD68 positive cells (red) staining and nuclei (dapi, blue) are showing for males (M) and females (N) at 1 dpi. Scale bars for images 50 μm, and for high-power magnification boxes 20μm. ** = p < 0.01; *** = p < 0.001 vs. females at the same time-point n = 5/group.

Article Snippet: For double labeling, sections were processed with polyclonal anti-rabbit P2Y12 (1:1000, Anaspec, San Jose, CA) and polyclonal anti-rat F4/80 (1:200, R&D Systems, Minneapolis, MN) overnight at 4°C.

Techniques: Staining